PVv3, a New Shuttle Vector for Gene Expression in Vibrio vulnificus
نویسندگان
چکیده
منابع مشابه
PVv3, a new shuttle vector for gene expression in Vibrio vulnificus.
An efficient electroporation procedure for Vibrio vulnificus was designed using the new cloning vector pVv3 (3,107 bp). Transformation efficiencies up to 2 × 10(6) transformants per μg DNA were achieved. The vector stably replicated in both V. vulnificus and Escherichia coli and was also successfully introduced into Vibrio parahaemolyticus and Vibrio cholerae. To demonstrate the suitability of ...
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The extracellular capsule polysaccharide (CPS) of Vibrio vulnificus is a primary virulence factor which allows survival of the bacteria in the human host. To study the genes involved in expression of the capsule, we generated mutants that lost the ability to produce CPS following the insertion of a minitransposon into the genome of an encapsulated, clinical strain of V. vulnificus. A genomic re...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2013
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.03720-13